ISBN online: 978-80-244-6049-9 | DOI: 10.5507/lf.22.24460499

Laboratory Techniques in Cellular and Molecular Medicine

Khushboo Agrawal (Ed.), Jan Bouchal (Ed.), Viswanath Das (Ed.), Jiří Drábek (Ed.), Petr Džubák (Ed.), Marián Hajdúch (Ed.), Karel Koberna (Ed.), Anna Ligasová (Ed.), Martin Mistrík (Ed.), Juan Batista de Sanctis (Ed.), Josef Srovnal Ed.), Anna Janošťáková (A.), Viswanath Das (A.), Natálie Kudlová (A.), Jana Stránská (A.), Renata Buriánová (A.), Jana Kotulová (A.), Ermin Schadich (A.), Martin Ondra (A.), Agáta Kubíčková (A.), Soňa Gurská (A.), Pavla Flodrová (A.), Patrik Flodr (A.), Daniela Kurfürstová (A.), Gabriela Kořínková (A.), Mariam Gachechiladze (A.), Tereza Buchtová (A.), Martin Mistrík (A.), Radek Trojanec (A.), Zuzana Šporiková (A.), Jiří Řehulka (A.), Anna Ligasová (A.), Karel Koberna (A.), Jarmila Stanková (A.), Ivo Frydrych (A.), Khushboo Agrawal (A.), Matthew Lacey (A.), Zdeněk Škrott (A.), Vlastimil Mašek (A.), Martina Medvedíková (A.), Ján Gurský (A.), Petr Džubák (A.), Juan B. De Sanctis (A.), Lenka Hrubá (A.), Jana Václavková (A.), Dušan Holub (A.), Tomáš Oždian (A.), Martina Jakoubková (A.), Miloš Petřík (A.), Zbyněk Nový (A.), Narendran Annadurai (A.), Alejandro Carazo (A.), Barbora Lišková (A.), Vladimíra Koudeláková (A.), Vaishali Uniyal (A.), Jiří Drábek (A.), Zuzana Macečková (A.), Hanuš Slavík (A.), Josef Srovnal (A.), Alona Řehulková (A.), Karolína Bartáková (A.), Rastislav Slavkovský (A.)

The methodological book "Laboratory techniques in cellular and molecular medicine" is intended for students of bachelor, master, and doctoral study programmes at faculties of science, medicine, and veterinary medicine, as well as for laboratory technicians interested in methodological approaches of contemporary cellular and molecular medicine. The book does not aim to provide a comprehensive overview of the current state of the art in cellular and molecular medicine, that would be a superhuman task. The aim of the 56-member author team is to provide readers with an overview of the methods established and tested at the Institute of Molecular and Translational Medicine of the Faculty of Medicine of Palacký University Olomouc – to describe the methodological principles and their practical application. It focuses both on basic methods, whose principles are used by the most modern methods, and on special methods, reflecting the laboratory experience and specialisation of individual authors. The 52 chapters describe the work with cells and microorganisms, bioengineering manipulations of nucleic acids, the search for biomarkers, detection at the level of DNA, RNA, protein, organelle, and whole cell, and the use of fluorescent and radioactive labeling. To aid comprehension, the description of the methods is illustrated by figures and diagrams. Each chapter is followed by troubleshooting tips. The book promotes the best laboratory practice to increase the reproducibility of results in biomedicine.

1. edition, online: 2022, publisher: Univerzita Palackého v Olomouci, Křížkovského 8, 771 47 Olomouc