DOI: 10.5507/vup.21.24459677.14

Pooled sequencing reveals genome regions involved in resistance to bacterial wilt in Italian ryegrass

Florian Goettelmann, Dario Copetti, Steven Yates, Bruno Studer, Roland Kölliker

Xanthomonas translucens pv. graminis (Xtg) is the causal agent of bacterial wilt, one of the main diseases of Italian ryegrass (Lolium multiflorum Lam.). One major QTL for resistance was previously discovered, but the underlying genes are yet to be determined. In order to fine-map this QTL, a mapping population consisting of 7,484 F2 individuals segregating for resistance was established in the greenhouse and inoculated with a highly virulent Xtg strain. Two pools of the most resistant and the most susceptible individuals were sequenced and SNPs associated with resistance were identified. Most of the significant SNPs map to linkage group 4, where the QTL was previously identified. Genes containing these SNPs will be determined and will constitute candidate resistance genes to be investigated further.

pages: 59-62



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